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Single-particle cryo-EM unaligned micrographs of Sevenless extracellular domain (pH 6.6)

ABSTRACT:

SUBMITTER: Cerutti Gabriele

PROVIDER: EMPIAR-12304 | biostudies-other |

REPOSITORIES: biostudies-other

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Structures and pH-dependent dimerization of the sevenless receptor tyrosine kinase.

Cerutti Gabriele G Arias Ronald R Bahna Fabiana F Mannepalli Seetha S Katsamba Phinikoula S PS Ahlsen Goran G Kloss Brian B Bruni Renato R Tomlinson Andrew A Shapiro Lawrence L

Molecular cell 20241106 23

Sevenless (Sev) is a Drosophila receptor tyrosine kinase (RTK) required for the specification of the R7 photoreceptor. It is cleaved into α and β subunits and binds the ectodomain of the G-protein-coupled receptor bride of sevenless (Boss). Previous work showed that the Boss ectodomain could bind but not activate Sev; rather, the whole seven-pass transmembrane Boss was required. Here, we show that Sev does not need to be cleaved to function and that a single-pass transmembrane form of Boss activ ...[more]

PMID: 39510067

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