Project description:Stroke is a highly prevalent and devastating disease with limited therapeutic options. Most efforts to develop treatments for stroke have thus far met with limited success in large clinical trials. The brain has an enormous capacity for self-preservation, and elucidating how the brain protects itself may provide novel insights into stroke treatment. Ischemic tolerance (IT), a phenomenon in which application of sub-lethal stress [preconditioning (PC) stimulus; PC] induces a state of tolerance to a subsequent ischemic insult, represents an example of endogenous neuroprotection. IT can be induced in brain by systemic administration of the Toll-like receptor-4 ligand lipopolysaccharide (LPS). Different populations of immune cell could potentially play a role in PC induced by LPS. In particular, monocytes, which are the main target of LPS, can exert a powerful protective effect either directly or by modulating the immune system.
Project description:In ischemic stroke, DWI-T2 mismatch (positive signals on DWI but negative signals on T2) indicates ischemia within 4.5 h. However, the molecular expression pattern of this region remains elusive. This project aimed to reveal the proteomics profiling of the brain tissues at DWI-T2 mismatch, T2(+), and contralateral regions of brain within 4.5 h after middle cerebral artery occlusion compared with naïve brains of mice.