Functional metagenomic screening for anti-CRISPR acitvity

ABSTRACT: Using a synthetic genetic circuit we developed a high-throughput approach to discover anti-CRISPR genes from metagenomic libraries based on their functional activity rather than sequence homology or genetic context. We identified eleven DNA fragments from soil, animal and human metagenomes that circumvent Streptococcus pyogenes Cas9 activity in our selection strain. We transformed different metagenomic libraries into our selection strain and then selected the libraries in inhibitory concentrations of chloramphenicol (30 µg ml-1) and collected the clones that appeared on the selective plates. Metagenomic inserts were extracted from pooled clones on selection plates, barcoded per library and sequenced using nanopore technology to obtain full inserts. Note that this submission is linked to European Nucleotide Archive Study ID PRJEB29470, where the sequences of the four anti-CRISPRs discovered in this study have been submitted.

SUBMITTER: Maria-Anna Misiakou 

PROVIDER: S-BSST226 | biostudies-other |

SECONDARY ACCESSION(S): PRJEB29470

REPOSITORIES: biostudies-other