Hyperbranched poly(-lysine) substrate presenting the laminin sequence YIGSR induces the formation of spheroids in adult bone marrow stem cells

ABSTRACT: Unlike the fibroblast-like cells formed upon monolayer culture of human mesenchymal stem cells, the natural stem cell niche of the bone marrow and other types of tissues favours the formation of 3-dimensional (3D) cell clusters. The structuring and biological activity of these clusters are regulated by the contacts established by cells with both the basement membrane and neighbour cells and results in their asymmetric division and the consequent maintenance of both a stem population and a committed progeny. The present work demonstrates the potential of a synthetic substrate to mimic the stem cell niche in vitro. The side amino groups of a linear Poly-L-lysine were modified with hyperbranched poly-(-lysine) peptides, named as dendrons, tethered with the lamininmimicking sequence, YIGSR. Th

SUBMITTER: Valeria Perugini 

PROVIDER: S-BSST59 | biostudies-other |

REPOSITORIES: biostudies-other