A 5? Sequence of Human HMGB2 Gene for Transcriptional Targeting of Glioblastoma

ABSTRACT: Achievement of specific tumor cell targeting remains a challenge for glioma gene therapy. We report here the identification and characterization of a 5’ sequence of human HMGB2 gene for transcriptional targeting to glioblastoma. We performed microarray analysis and found HMGB2 as one of the genes that had a low level of expression in normal human astrocytes, but was significantly up-regulated in glioblastoma cells. Real-time PCR quantification revealed increase in HMBG2 expression level in glioblastoma tissues and cells between 11 to 79 fold over that in normal human brain tissue. With progressive truncation of a 5’-upstream sequence of the HMGB2 gene, we identified a 500-bp fragment that displayed a high transcriptional activity in glioblastoma cells, but a low activity in normal brain ce

ORGANISM(S): Homo sapiens

SUBMITTER: Boulaire Jerome 

PROVIDER: S-ECPF-GEOD-12305 | biostudies-other |

REPOSITORIES: biostudies-other