Unique signatures of estrogen independent growth of MCF-7 cells in low-estrogen versus complete estrogen depravation

ABSTRACT: "Despite the success of the aromatase inhibitors (AIs) in treating estrogen receptor positive breast cancer, 15-20% of patients receiving adjuvant AIs will relapse within 5-10 years of treatment initiation. Long term estrogen deprivation (LTED) of breast cancer cells in culture has been successfully used to mimic AI-induced estrogen depletion to dissect mechanisms of AI resistance. However, we hypothesized that a subset of patients receiving AI therapy may maintain low circulating concentrations of estrogens that influence the development of endocrine resistance. We sought to expand established LTED models to account for these observations. MCF-7 cells were grown in medium with charcoal stripped serum supplemented with defined concentrations of E2 or the estrogenic androgen metabolite 3βAd

ORGANISM(S): Homo sapiens

SUBMITTER: Strumba Viktoriya 

PROVIDER: S-ECPF-GEOD-33287 | biostudies-other |

REPOSITORIES: biostudies-other