Transcription profiling of human mammary epithelial cells over-expressing AP2

ABSTRACT: BACKGROUND: The AP2 transcription factor family is a set of developmentally regulated, retinoic acid (RA) inducible genes, which regulate expression of estrogen receptor-alpha (ERalpha) in breast carcinoma. We hypothesized that AP2 factors regulate a set of genes characteristic of the hormone responsive breast cancer phenotype. To better understand the role of AP2 factors in hormone responsive breast cancer, we sought to identify AP2-target genes in breast epithelial cells. MATERIALS AND METHODS: Overexpression of AP2 factors was achieved in human mammary epithelial cells (HMECs) using adenoviral vectors. AP2 target genes were identified by comparative hybridization to cDNA microarrays containing 30,000 human genes. Expression patterns were confirmed by Northern and Western blot and by eli

ORGANISM(S): Homo sapiens

SUBMITTER: McPherson LA 

PROVIDER: S-ECPF-SMDB-4003 | biostudies-other | 2007 Mar

REPOSITORIES: biostudies-other