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Induction of antigen-specific regulatory T cells in wild-type mice: visualization and targets of suppression.

ABSTRACT: Antigen-specific transplantation tolerance in the absence of immunosuppressive drugs is a rarely achieved goal. Immune responses to Y chromosome-encoded transplantation antigens (HY) can have life-threatening consequences in the clinic. Here, we have adopted a procedure developed in T cell antigen receptor (TCR)-transgenic mice to convert naïve T cells into male-specific Foxp3(+) regulatory T cells (Tregs) in WT female mice. For this purpose, female mice were infused by osmotic minipumps with a single class II MHC-presented HY peptide and Tregs visualized by tetramer staining. As a result, animals developed Treg-mediated long-term tolerance to all HY transplantation antigens, irrespective of whether they were recognized by CD4 or CD8 T cells, on skin or hematopoietic grafts from male donors.

SUBMITTER: Verginis P

PROVIDER: S-EPMC2265184 | biostudies-other | 2008 Mar

REPOSITORIES: biostudies-other

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Induction of antigen-specific regulatory T cells in wild-type mice: visualization and targets of suppression.

Verginis Panayotis P McLaughlin Katherine A KA Wucherpfennig Kai W KW von Boehmer Harald H Apostolou Irina I

Proceedings of the National Academy of Sciences of the United States of America 20080225 9

Antigen-specific transplantation tolerance in the absence of immunosuppressive drugs is a rarely achieved goal. Immune responses to Y chromosome-encoded transplantation antigens (HY) can have life-threatening consequences in the clinic. Here, we have adopted a procedure developed in T cell antigen receptor (TCR)-transgenic mice to convert naïve T cells into male-specific Foxp3(+) regulatory T cells (Tregs) in WT female mice. For this purpose, female mice were infused by osmotic minipumps with a ...[more]

PMID: 18299571

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