Ontology highlight
ABSTRACT:
SUBMITTER: Lee CH
PROVIDER: S-EPMC265602 | biostudies-other | 1993 Jun
REPOSITORIES: biostudies-other
Lee C H CH Bauer N L NL Shaw M M MM Durkin M M MM Bartlett M S MS Queener S F SF Smith J W JW
Journal of clinical microbiology 19930601 6
A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites. ...[more]