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Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.

ABSTRACT: A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

SUBMITTER: Lee CH

PROVIDER: S-EPMC265602 | biostudies-other | 1993 Jun

REPOSITORIES: biostudies-other

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Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.

Lee C H CH Bauer N L NL Shaw M M MM Durkin M M MM Bartlett M S MS Queener S F SF Smith J W JW

Journal of clinical microbiology 19930601 6

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites. ...[more]

PMID: 8315014

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