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Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.

ABSTRACT: We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.

SUBMITTER: Timmers HT 

PROVIDER: S-EPMC330199 | biostudies-other | 1990 Jan

REPOSITORIES: biostudies-other

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Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.

Timmers H T HT   Pronk G J GJ   Bos J L JL   van der Eb A J AJ  

Nucleic acids research 19900101 1

We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1  ...[more]