The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits.

ABSTRACT: BACKGROUND: Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation. RESULTS: TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits. CONCLUSIONS: TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.

SUBMITTER: Hoogeveen-Westerveld M 

PROVIDER: S-EPMC3517901 | biostudies-other | 2012

REPOSITORIES: biostudies-other