Ontology highlight
ABSTRACT:
SUBMITTER: Ding Q
PROVIDER: S-EPMC3570604 | biostudies-other | 2013 Feb
REPOSITORIES: biostudies-other
Cell stem cell 20121213 2
Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are easier to design to cleave at desired sites in a genome than previous types of nucleases. We report here the use of TALENs to rapidly and efficiently generate mutant alleles of 15 genes in cultured somatic cells or human pluripotent stem cells, the latter for which we differentiated both the targeted lines and isogenic control lines into various metabolic cell types. We demonstrate cell-auto ...[more]