Unknown

Dataset Information

0

Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.

ABSTRACT: We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

SUBMITTER: Tzur YB 

PROVIDER: S-EPMC3813848 | biostudies-other | 2013 Nov

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.

Tzur Yonatan B YB   Friedland Ari E AE   Nadarajan Saravanapriah S   Church George M GM   Calarco John A JA   Colaiácovo Monica P MP  

Genetics 20130826 3

We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans. ...[more]

Similar Datasets

Unknown Efficient marker-free recovery of custom genetic modifications with CRISPR/Cas9 in Caenorhabditis elegans.
| S-EPMC4224173 | biostudies-literature
Unknown Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
| S-EPMC3822328 | biostudies-literature
Unknown CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.
| S-EPMC3813849 | biostudies-other
Unknown Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins.
| S-EPMC3813847 | biostudies-other
Unknown Targeted Chromosomal Rearrangements via Combinatorial Use of CRISPR/Cas9 and Cre/LoxP Technologies in Caenorhabditis elegans.
| S-EPMC6071600 | biostudies-other
Unknown Highly Efficient and Heritable Targeted Mutagenesis in Wheat via the Agrobacterium tumefaciens-Mediated CRISPR/Cas9 System.
| S-EPMC6747105 | biostudies-literature
Unknown Heat-shock-inducible CRISPR/Cas9 system generates heritable mutations in rice.
| S-EPMC6603394 | biostudies-literature
Unknown CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans.
| S-EPMC6325907 | biostudies-literature
Unknown Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans.
| S-EPMC4273605 | biostudies-literature
Unknown Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9.
| S-EPMC5030659 | biostudies-literature