Identification of IFN-?-producing innate B cells.

ABSTRACT: Although B cells play important roles in the humoral immune response and the regulation of adaptive immunity, B cell subpopulations with unique phenotypes, particularly those with non-classical immune functions, should be further investigated. By challenging mice with Listeria monocytogenes, Escherichia coli, vesicular stomatitis virus and Toll-like receptor ligands, we identified an inducible CD11a(hi)Fc?RIII(hi) B cell subpopulation that is significantly expanded and produces high levels of IFN-? during the early stage of the immune response. This subpopulation of B cells can promote macrophage activation via generating IFN-?, thereby facilitating the innate immune response against intracellular bacterial infection. As this new subpopulation is of B cell origin and exhibits the phenotypic characteristics of B cells, we designated these cells as IFN-?-producing innate B cells. Dendritic cells were essential for the inducible generation of these innate B cells from the follicular B cells via CD40L-CD40 ligation. Increased Bruton's tyrosine kinase activation was found to be responsible for the increased activation of non-canonical NF-?B pathway in these innate B cells after CD40 ligation, with the consequent induction of additional IFN-? production. The identification of this new population of innate B cells may contribute to a better understanding of B cell functions in anti-infection immune responses and immune regulation.

SUBMITTER: Bao Y 

PROVIDER: S-EPMC3915900 | biostudies-other | 2014 Feb

REPOSITORIES: biostudies-other