Ontology highlight
ABSTRACT:
SUBMITTER: Gong H
PROVIDER: S-EPMC5459813 | biostudies-other | 2017 Jun
REPOSITORIES: biostudies-other
Scientific reports 20170605 1
We have investigated the correlation between proteins and mRNAs in single cells employing an integrated workflow for dual-analyte co-detection. This is achieved by combining the oligo extension reaction (OER), which converts protein levels to DNA levels, with reverse transcription for mRNA detection. Unsupervised gene expression profiling analysis, including principal component analysis and hierarchical clustering, revealed different aspects of the protein-mRNA relationship. Violin plot analysis ...[more]