Unknown

Dataset Information

0

Assessment of Single Cell RNA-Seq Normalization Methods.

ABSTRACT: We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in External RNA Control Consortium (ERCC) RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data.

SUBMITTER: Ding B 

PROVIDER: S-EPMC5499114 | biostudies-other | 2017 Jul

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Assessment of Single Cell RNA-Seq Normalization Methods.

Ding Bo B   Zheng Lina L   Wang Wei W  

G3 (Bethesda, Md.) 20170705 7

We have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Our analyses showed that methods considering spike-in External RNA Control Consortium (ERCC) RNA molecules significantly outperformed those not considering ERCCs. This work provides a guidance of selecting normalization methods to remove technical noise in single cell RNA-seq data. ...[more]

Similar Datasets

Unknown Normalization Methods on Single-Cell RNA-seq Data: An Empirical Survey.
| S-EPMC7019105 | biostudies-literature
Unknown Performance Assessment and Selection of Normalization Procedures for Single-Cell RNA-Seq.
| S-EPMC6544759 | biostudies-literature
Unknown SCnorm: robust normalization of single-cell RNA-seq data.
| S-EPMC5473255 | biostudies-literature
Unknown Normalization and noise reduction for single cell RNA-seq experiments.
| S-EPMC4481848 | biostudies-literature
Unknown PsiNorm: a scalable normalization for single-cell RNA-seq data.
| S-EPMC8696108 | biostudies-literature
Unknown Non-linear Normalization for Non-UMI Single Cell RNA-Seq.
| S-EPMC8063035 | biostudies-literature
Unknown Analytic Pearson residuals for normalization of single-cell RNA-seq UMI data.
| S-EPMC8419999 | biostudies-literature
Unknown The Impact of Normalization Methods on RNA-Seq Data Analysis.
| S-EPMC4484837 | biostudies-literature
Unknown Evaluation of Normalization Methods for RNA-Seq Gene Expression Estimation.
| S-EPMC4983420 | biostudies-literature
Unknown Quantile normalization of single-cell RNA-seq read counts without unique molecular identifiers.
| S-EPMC7333325 | biostudies-literature