Dataset Information

Pilot Study for The Presence of Fungal Metabolites in Sheep Milk from First Spring Milking.

ABSTRACT: Introduction:A mini-study of 20 raw milk samples was conducted to examine the spectrum of fungal metabolites in sheep milk from the first spring milking. Material and Methods:Samples were collected from randomly selected ewes in two animal flocks from the Bieszczady Mountains and analysed using liquid chromatography-tandem mass spectrometry. Results:Out of ~700 bacterial, fungal, and plant metabolites tested for, only one mycotoxin - Enniatin B - was detected in sheep milk samples (18/20; 0.0055-0.0121 μg/kg; 0.0078 μg/kg average). Conclusions:The results indicated that there was no high-level exposure to fungal metabolites via consumption of raw sheep milk during the sample collection period.

SUBMITTER: Piatkowska M

PROVIDER: S-EPMC6200299 | biostudies-other | 2018 Jun

REPOSITORIES: biostudies-other

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Pilot Study for The Presence of Fungal Metabolites in Sheep Milk from First Spring Milking.

Piątkowska Marta M Sulyok Michael M Pietruszka Katarzyna K Panasiuk Łukasz Ł

Journal of veterinary research 20180601 2

<h4>Introduction</h4>A mini-study of 20 raw milk samples was conducted to examine the spectrum of fungal metabolites in sheep milk from the first spring milking.<h4>Material and methods</h4>Samples were collected from randomly selected ewes in two animal flocks from the Bieszczady Mountains and analysed using liquid chromatography-tandem mass spectrometry.<h4>Results</h4>Out of ~700 bacterial, fungal, and plant metabolites tested for, only one mycotoxin - Enniatin B - was detected in sheep milk ...[more]

PMID: 30364877

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Project description:BackgroundIn order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution and target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) and 35 randomly sampled from two abattoirs (B) together with five negative controls (C). An immunocytochemistry technique was developed and further milk samples (n = 39) were used to study viral excretion, carrier cells and the role of milk and colostrum in the transmission of the disease.ResultsAll sheep from group C and three sheep from group B were negative to VM in tissue sections by histopathology, immunohistochemistry and PCR, and also in serum using ELISA. Several degrees of CD3 + lymphocytic interstitial mastitis were observed in groups A and B: minimal (+) n = 26 sheep; moderate (++), n = 32 and severe (+++), n = 12. No differences in lesion distribution were observed between groups A and B. Viral presence was confirmed by immunohistochemistry using two different antibodies and/or PCR in every tissue with lesions while serology was negative in six sheep with lesions. Two milk samples taken from milk tanks from two flocks from group A and fourteen milk samples from 29 infected sheep from group B were positive to VM (most of them from animals with moderate and severe lesions). Positivity was only found in macrophages, even in focal and minimal lesions, while no positivity was observed in epithelial or any other cells in either tissue and milk samples.ConclusionsThis new observation of the minimal lesions described in this work increased the prevalence of VM lesions in mammary gland up to 90.9% and VM should be considered as a differential diagnosis when minimal interstitial lesions are detected. A high prevalence of VM was observed in intensive milk-producing sheep, ELISA serology did not detect as positivity all infected animals, while histology, IHC or PCR showed higher sensitivity. The cytological technique developed was very useful in milk-cell studies using hematoxylin and eosin and immunocytochemistry. Viral detection in milk samples (16/39) confirms a potential but limited role of milk/colostrum in viral transmission.

Dataset Information

Pilot Study for The Presence of Fungal Metabolites in Sheep Milk from First Spring Milking.

Publications

Pilot Study for The Presence of Fungal Metabolites in Sheep Milk from First Spring Milking.

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