Dataset Information

UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin.

ABSTRACT: Phenolic compounds represent a large class of secondary metabolites, involved in multiple functions not only in plant life cycle, but also in fruit during post-harvest. phenolics play a key role in the response to biotic and abiotic stresses, thus their accumulation is regulated by the presence of environmental stimuli. The present work aimed to investigate how different pre-UV-B-exposures can modulate the phenolic response of peach fruit infected with Monilinia fructicola. Through HPLC-DAD-MSn, several procyanidins, phenolic acids, flavonols, and anthocyanins were detected. Both UV-B radiation and fungal infection were able to stimulate the accumulation of phenolics, dependent on the chemical structure. Regarding UV-B exposure, inoculated with sterile water, 3 h of UV-B radiation highest concentration of phenolics was found, especially flavonols and cyanidin-3-glucoside far from the wound. However, wounding decreased the phenolics in the region nearby. When peaches were pre-treated with 1 h of UV-B radiation, the fungus had an additive effect in phenolic accumulation far from the infection, while it had a subtractive effect with 3 h of UV-B radiation, especially for flavonols. Canonical discriminant analysis and Pearson correlation revealed that all phenolic compounds, except procyanidin dimer, were highly regulated by UV-B radiation, with particularly strong correlation for quercetin and kaempferol glycosides, while phenolics correlated with the fungus infection were quercetin-3-galactoside, quercetin-3-glucoside, kaempferol-3-galactoside and isorhamnetin-3-glucoside. Modulation of pathogen-induced phenolics also far from inoculation site might suggest a migration of signaling molecules from the infected area to healthy tissues.

SUBMITTER: Santin M

PROVIDER: S-EPMC6233700 | biostudies-other | 2018

REPOSITORIES: biostudies-other

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UV-B Pre-treatment Alters Phenolics Response to <i>Monilinia fructicola</i> Infection in a Structure-Dependent Way in Peach Skin.

Santin Marco M Neugart Susanne S Castagna Antonella A Barilari Martina M Sarrocco Sabrina S Vannacci Giovanni G Schreiner Monika M Ranieri Annamaria A

Frontiers in plant science 20181106

Phenolic compounds represent a large class of secondary metabolites, involved in multiple functions not only in plant life cycle, but also in fruit during post-harvest. phenolics play a key role in the response to biotic and abiotic stresses, thus their accumulation is regulated by the presence of environmental stimuli. The present work aimed to investigate how different pre-UV-B-exposures can modulate the phenolic response of peach fruit infected with <i>Monilinia fructicola</i>. Through HPLC-D ...[more]

PMID: 30459788

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Project description:Recent global radiation fears reflect the urgent need for a new modality that can simply determine if people are in a radiation risk of developing cancer and other illnesses. Ultraviolet (UV) radiation has been thought to be the major risk factor for most skin cancers. Although various biomarkers derived from the responses of human cells have been revealed, detection of these biomarkers is cumbersome, probably requires taking live human tissues, and varies significantly depending on human immune status. Here we hypothesize that the reaction of Propionibacterium acnes (P. acnes), a human resident skin commensal, to UV radiation can serve as early surrogate markers for radiation risk because the bacteria are immediately responsive to radiation. In addition, the bacteria can be readily accessible and exposed to the same field of radiation as human body. To test our hypothesis, P. acnes was exposed to UV-B radiation. The production of porphyrins in P. acnes was significantly reduced with increasing doses of UV-B. The porphyrin reduction can be detected in both P. acnes and human skin bacterial isolates. Exposure of UV-B to P. acnes- inoculated mice led to a significant decrease in porphyrin production in a single colony of P. acnes and simultaneously induced the formation of cyclobutane pyrimidine dimers (CPD) in the epidermal layers of mouse skin. Mass spectrometric analysis via a linear trap quadrupole (LTQ)-Orbitrap XL showed that five peptides including an internal peptide (THLPTGIVVSCQNER) of a peptide chain release factor 2 (RF2) were oxidized by UV-B. Seven peptides including three internal peptides of 60 kDa chaperonin 1 were de-oxidized by UV-B. When compared to UV-B, gamma radiation also decreased the porphyrin production of P. acnes in a dose-dependent manner, but induced a different signature of protein oxidation/de-oxidation. We highlight that uncovering response of skin microbiome to radiation will facilitate the development of pre-symptomatic diagnosis of radiation risk in a battlefield exposure, nuclear accidents, terrorist attacks, or cancer imaging/therapy.

Dataset Information

UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin.

Publications

UV-B Pre-treatment Alters Phenolics Response to <i>Monilinia fructicola</i> Infection in a Structure-Dependent Way in Peach Skin.

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