Systematic identification of structure-specific protein-protein interactions

ABSTRACT: The physical interactome of a protein can be altered upon perturbation, modulating cell physiology and contributing to disease. Interactome differences of normal and disease states of proteins could explain disease mechanisms, but current methods do not pinpoint structure-specific PPIs and interaction interfaces proteome-wide. We used limited proteolysis-mass spectrometry (LiP-MS) to screen for structure-specific PPIs by probing for protein structural alterations in cellular extracts upon treatment with specific structural states of a protein. We demonstrated that LiP-MS detects well-characterized PPIs, including antibody-target interactions and interactions with membrane proteins, and pinpoints interfaces. We then studied structure-specific interactors of the Parkinson's disease hallmark

SUBMITTER: Dr Aleš Holfeld 

PROVIDER: S-SCDT-10_1038-S44320-024-00037-6 | biostudies-other |

REPOSITORIES: biostudies-other