Glycolysis regulates Hedgehog signaling via the plasma membrane potential

ABSTRACT: Changes in cell metabolism and plasma membrane potential have been linked to shifts between tissue growth and differentiation, and to developmental patterning. How such changes mediate these effects is not poorly understood. Here, we use the developing wing of Drosophila to investigate the interplay between cell metabolism and a key developmental regulator - the Hedgehog (Hh) signaling pathway. We show that reducing glycolysis both lowers steady-state levels of ATP and stabilizes Smoothened (Smo), the 7-pass transmembrane protein that transduces the Hh signal. As a result, the transcription factor Cubitus interruptus accumulates in its full-length, transcription activating form. We show that glycolysis is required to maintain the plasma membrane potential and that plasma membrane depolariz

SUBMITTER: Dr. Stephanie Spannl 

PROVIDER: S-SCDT-EMBOJ-2019-101767 | biostudies-other |

REPOSITORIES: biostudies-other