A single-cell RNA expression atlas of normal, preneoplastic and tumorigenic states in the human breast

ABSTRACT: To examine global changes in breast heterogeneity across different states, we determined the single-cell transcriptomes of >340,000 cells encompassing normal breast, preneoplastic BRCA1+/- tissue, the major breast cancer subtypes, and pairs of tumors and involved lymph nodes. Elucidation of the normal breast microenvironment revealed striking changes in the stroma of postmenopausal women. Single-cell profiling of 34 treatment-naive primary tumors, including estrogen receptor (ER)+, HER2+ and triple negative breast cancers, revealed comparable diversity amongst cancer cells and a discrete subset of cycling cells. The transcriptomes of preneoplastic BRCA1+/- tissue versus tumors highlighted global changes in the immune microenvironment. Within the tumor immune landscape, proliferative CD8

SUBMITTER: Dr. Bhupinder Pal 

PROVIDER: S-SCDT-EMBOJ-2020-107333 | biostudies-other |

REPOSITORIES: biostudies-other