HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell-to-cell and vascular microRNA movement

ABSTRACT: Plant microRNAs (miRNAs) guide cytosolic post-transcriptional-gene-silencing of sequence-complementary transcripts within the producing cell, as well as in distant cells and tissues. Here, we used an artificial miRNA-based system (amiRSUL) in Arabidopsis thaliana to explore the still elusive mechanisms of inter-cellular miRNA movement via forward genetics. This screen identified many mutant alleles of HASTY (HST), the ortholog of mammalian EXPORTIN5 (XPO5) with a recently reported role in miRNA biogenesis. In both epidermis-peeling and grafting assays, amiRSUL levels were reduced more substantially in miRNA-recipient tissues than in silencing-emitting tissues, indicating a HST role in controlling direct cell-to-cell and phloem-mediated movement of the processed amiRSUL, in addition to regu

SUBMITTER: Dr. Florian BRIOUDES 

PROVIDER: S-SCDT-EMBOJ-2020-107455P | biostudies-other |

REPOSITORIES: biostudies-other