Genomics

Dataset Information

41

NextGen Consortium: Globin Gene Expression in Sickle Cell Genotype-Specific iPS cells


ABSTRACT: 1. Implement an efficient, highly reproducible and 'scalable' system for the production of large numbers of sickle cell anemia-specific iPS cells (iPSC). 2. Derive and characterize a novel, in vitro system for the production of an unlimited supply of erythroid lineage cells from the directed differentiation of 'clinical grade' transgene-free iPS cells; use this system to recapitulate erythroid-lineage ontogeny in vitro with the sequential development of primitive and definitive erythropoiesis, accompanied by the appropriate expression of stage-specific globin genes. 3. Identify developmental gene expression profile differences between erythroid precursors that produce primarily HbF and those that produce primarily HbA or HbS. 4. Determine the effects of the three known HbF major quantitative trait loci (QTL) on globin gene expression in disease-specific iPS cells during in vitro erythropoiesis. 5. Search for novel HbF genetic modifiers associated... (for more see dbGaP study page.)

PROVIDER: phs001212 | dbGaP |

SECONDARY ACCESSION(S): PRJNA353151PRJNA353152

REPOSITORIES: dbGaP

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