Ontology highlight
ABSTRACT: We used massively parallel, paired-end sequencing of expressed transcripts (RNA-seq) to detect novel gene fusions in short-term cultures of glioma stem-like cells freshly isolated from nine patients carrying primary glioblastoma multiforme (GBM). The culture of primary GBM tumors under serum-free conditions selects cells that retain phenotypes and genotypes closely mirroring primary tumor profiles as compared to serum-cultured glioma cell lines that have largely lost their developmental identities.
OTHER RELATED OMICS DATASETS IN: PRJNA94461
PROVIDER: phs000505.v1.p1 | EGA |
REPOSITORIES: EGA
Furnari Frank B FB Fenton Tim T Bachoo Robert M RM Mukasa Akitake A Stommel Jayne M JM Stegh Alexander A Hahn William C WC Ligon Keith L KL Louis David N DN Brennan Cameron C Chin Lynda L DePinho Ronald A RA Cavenee Webster K WK
Genes & development 20071101 21
Malignant astrocytic gliomas such as glioblastoma are the most common and lethal intracranial tumors. These cancers exhibit a relentless malignant progression characterized by widespread invasion throughout the brain, resistance to traditional and newer targeted therapeutic approaches, destruction of normal brain tissue, and certain death. The recent confluence of advances in stem cell biology, cell signaling, genome and computational science and genetic model systems have revolutionized our und ...[more]