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Dataset Information


Gene fusion discovery through RNA sequencing of nine human glioblastoma stem cell lines


We used massively parallel, paired-end sequencing of expressed transcripts (RNA-seq) to detect novel gene fusions in short-term cultures of glioma stem-like cells freshly isolated from nine patients carrying primary glioblastoma multiforme (GBM). The culture of primary GBM tumors under serum-free conditions selects cells that retain phenotypes and genotypes closely mirroring primary tumor profiles as compared to serum-cultured glioma cell lines that have largely lost their developmental identities.


ORGANISM(S): Homo sapiens  

PROVIDER: phs000505.v1.p1 | EGA |


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Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.

Lee Jeongwu J   Kotliarova Svetlana S   Kotliarov Yuri Y   Li Aiguo A   Su Qin Q   Donin Nicholas M NM   Pastorino Sandra S   Purow Benjamin W BW   Christopher Neil N   Zhang Wei W   Park John K JK   Fine Howard A HA  

Cancer cell 20060501 5

The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural  ...[more]

Publication: 1/2

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