Genomics

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Gene fusion discovery through RNA sequencing of nine human glioblastoma stem cell lines


ABSTRACT:

We used massively parallel, paired-end sequencing of expressed transcripts (RNA-seq) to detect novel gene fusions in short-term cultures of glioma stem-like cells freshly isolated from nine patients carrying primary glioblastoma multiforme (GBM). The culture of primary GBM tumors under serum-free conditions selects cells that retain phenotypes and genotypes closely mirroring primary tumor profiles as compared to serum-cultured glioma cell lines that have largely lost their developmental identities.

OTHER RELATED OMICS DATASETS IN: E-GEOD-4536PRJNA94461

PROVIDER: phs000505.v1.p1 | EGA |

REPOSITORIES: EGA

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Publications

Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.

Lee Jeongwu J   Kotliarova Svetlana S   Kotliarov Yuri Y   Li Aiguo A   Su Qin Q   Donin Nicholas M NM   Pastorino Sandra S   Purow Benjamin W BW   Christopher Neil N   Zhang Wei W   Park John K JK   Fine Howard A HA  

Cancer cell 20060501 5


The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural  ...[more]

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