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Characterization of the Gut-Associated Microbiome in Inflammatory Pouch Complications Following Ileal Pouch-Anal Anastomosis

ABSTRACT:

This study involved evaluation of the tissue associated microbiome of the ileal pouch following surgery for ulcerative colitis (UC) or familial adenomatous polyposis (FAP). Individuals were recruited, with biopsies taken from the ileal pouch and the pre-pouch ileum, microbial DNA was extracted and sequenced using 454 pyrosequencing. Total bacterial community structure and abundance were evaluated to determine which changes were characteristic of inflammatory phenotypes including pouchitis (inflammation of the ileal pouch) and a Crohn's disease-like phenotype.

PROVIDER: phs000659.v1.p1 | EGA |

REPOSITORIES: EGA

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Characterization of the gut-associated microbiome in inflammatory pouch complications following ileal pouch-anal anastomosis.

Tyler Andrea D AD Knox Natalie N Kabakchiev Boyko B Milgrom Raquel R Kirsch Richard R Cohen Zane Z McLeod Robin S RS Guttman David S DS Krause Denis O DO Silverberg Mark S MS

PloS one 20130924 9

<h4>Introduction</h4>Inflammatory complications following ileal pouch-anal anastomosis (IPAA) for ulcerative colitis (UC) are common and thought to arise through mechanisms similar to de novo onset inflammatory bowel disease. The aim of this study was to determine whether specific organisms in the tissue-associated microbiota are associated with inflammatory pouch complications.<h4>Methods</h4>Patients having previously undergone IPAA were recruited from Mount Sinai Hospital. Clinical and demogr ...[more]

PMID: 24086242

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