Transcriptomics,Genomics

Dataset Information

43

Gene expression profiles of mouse embryonic fibroblasts (MEFs), induced cardiomyocytes (iCMs) and heart.


ABSTRACT: Global gene expression patterns of the iCMs shift from a MEF state toward a cardiac-like phenotype by Gata4/Mef2c/Tbx5 (GMT) or GMT/Hand2 (GHMT) transduction at 2 and 4 weeks after transduction (2W, 4W). Hand2 upregulated a panel of cardiac genes and suppressed cell cylce genes during cardiac reprogramming. Overall design: MEFs were used for negative control, mouse heart tissue for positive control. Gene expression profiles were compared among MEFs, iCMs and heart.

INSTRUMENT(S): 3D-Gene Mouse Oligo chip 24k

SUBMITTER: Satoshi Kondo  

PROVIDER: GSE101578 | GEO | 2017-09-26

SECONDARY ACCESSION(S): PRJNA394871

REPOSITORIES: GEO

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Publications

Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression.

Umei Tomohiko C TC   Yamakawa Hiroyuki H   Muraoka Naoto N   Sadahiro Taketaro T   Isomi Mari M   Haginiwa Sho S   Kojima Hidenori H   Kurotsu Shota S   Tamura Fumiya F   Osakabe Rina R   Tani Hidenori H   Nara Kaori K   Miyoshi Hiroyuki H   Fukuda Keiichi K   Ieda Masaki M  

International journal of molecular sciences 20170819 8


Direct reprogramming is a promising approach in regenerative medicine. Overexpression of the cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) or GMT plus Hand2 (GHMT) directly reprogram fibroblasts into cardiomyocyte-like cells (iCMs). However, the critical timing of transgene expression and the molecular mechanisms for cardiac reprogramming remain unclear. The conventional doxycycline (Dox)-inducible temporal transgene expression systems require simultaneous transduction of two vector  ...[more]

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