Transcriptomics,Genomics

Dataset Information

25

Transcriptome analysis reveals molecular anthelmintic effects of procyanidins in C. elegans


ABSTRACT: Worldwide, more than 1 billion people are affected by infestations with soil-transmitted helminths and also in veterinary medicine helminthiases are a severe thread to livestock due to emerging resistances against the common anthelmintics. Proanthocyanidins have been increasingly investigated for their anthelmintic properties, however, except for an interaction with certain proteins of the nematodes, not much is known about their mode of action. To investigate the anthelmintic activity on a molecular level, a transcriptome analysis was performed in Caenorhabditis elegans after treatment with purified and fully characterized oligomeric procyanidins (OPC). The OPCs had previously been obtained from a hydro-ethanolic (1:1) extract from the leaves of Combretum mucronatum, a plant which is traditionally used in West Africa for the treatment of helminthiasis, therefore, also the crude extract was included in the study. Significant changes in differential gene expression were observed mainly for proteins related to the intestine, many of which were located extracellularly or within cellular membranes. Among the up-regulated genes, several hitherto undescribed orthologues of structural proteins in humans were identified, but also genes that are potentially involved in the worms’ defense against tannins. For example, T22D1.2, an orthologue of human basic salivary proline-rich protein (PRB) 2, and numr-1 (nuclear localized metal responsive) were found to be strongly up-regulated. Down-regulated genes were mainly associated with lysosomal activity, glycoside hydrolysis or the worms’ innate immune response. No major differences were found between the groups treated with purified OPCs versus the crude extract. Investigations using GFP reporter gene constructs of T22D1.2 and numr-1 corroborated the intestine as the predominant site of the anthelmintic activity. Overall design: 15 samples in total, 5 groups, 3 replicates each

INSTRUMENT(S): [EleGene-1_0-st] Affymetrix C. elegans Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Jochen Seggewiss 

PROVIDER: GSE101680 | GEO | 2017-08-31

SECONDARY ACCESSION(S): PRJNA395200

REPOSITORIES: GEO

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Publications

Transcriptome analysis reveals molecular anthelmintic effects of procyanidins in C. elegans.

Spiegler Verena V   Hensel Andreas A   Seggewiß Jochen J   Lubisch Milena M   Liebau Eva E  

PloS one 20170919 9


Worldwide, more than 1 billion people are affected by infestations with soil-transmitted helminths and also in veterinary medicine helminthiases are a severe threat to livestock due to emerging resistances against the common anthelmintics. Proanthocyanidins have been increasingly investigated for their anthelmintic properties, however, except for an interaction with certain proteins of the nematodes, not much is known about their mode of action. To investigate the anthelmintic activity on a mole  ...[more]

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