Transcriptomics,Genomics

Dataset Information

150

Nonmuscle myosin IIB regulates epicardial integrity and epicardium-derived mesenchymal cell maturation


ABSTRACT: Nonmuscle myosin IIB (NMIIB; heavy chain encoded by MYH10) is essential for cardiac myocyte cytokinesis. The role of NMIIB in other cardiac cells is not known. Here, we show that NMIIB is required in epicardial formation and functions to support myocardial proliferation and coronary vessel development. Ablation of NMIIB in epicardial cells results in disruption of epicardial integrity with a loss of E-cadherin at cell-cell junctions and a focal detachment of epicardial cells from the myocardium. NMIIB-knockout and blebbistatin-treated epicardial explants demonstrate impaired mesenchymal cell maturation during epicardial epithelial-mesenchymal transition. This is manifested by an impaired invasion of collagen gels by the epicardium-derived mesenchymal cells and the reorganization of the cytoskeletal structure. Although there is a marked decrease in the expression of mesenchymal genes, there is no change in Snail (also known as Snai1) or E-cadherin expression. Studies from epicardium-specific NMIIB-knockout mice confirm the importance of NMIIB for epicardial integrity and epicardial functions in promoting cardiac myocyte proliferation and coronary vessel formation during heart development. Our findings provide a novel mechanism linking epicardial formation and epicardial function to the activity of the cytoplasmic motor protein NMIIB. Overall design: Total RNA is prepared from 8 explants each with or without treatmnet with 20mM blebbistatin.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Yanqin Yang  

PROVIDER: GSE101701 | GEO | 2017-07-21

SECONDARY ACCESSION(S): PRJNA395226

REPOSITORIES: GEO

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Publications

Nonmuscle myosin IIB regulates epicardial integrity and epicardium-derived mesenchymal cell maturation.

Ma Xuefei X   Sung Derek C DC   Yang Yanqin Y   Wakabayashi Yoshi Y   Adelstein Robert S RS  

Journal of cell science 20170707 16


Nonmuscle myosin IIB (NMIIB; heavy chain encoded by MYH10) is essential for cardiac myocyte cytokinesis. The role of NMIIB in other cardiac cells is not known. Here, we show that NMIIB is required in epicardial formation and functions to support myocardial proliferation and coronary vessel development. Ablation of NMIIB in epicardial cells results in disruption of epicardial integrity with a loss of E-cadherin at cell-cell junctions and a focal detachment of epicardial cells from the myocardium.  ...[more]

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