Transcriptomics,Genomics

Dataset Information

48

Novel targets in injured cord in an obese SCI rat model


ABSTRACT: In the present study, we sought to understand the impact of obesity/metabolic disease (high-fat induced) on spinal cord injury (SCI) by examining transcriptome. Adult, male Long Evans rats received either thoracic level contusion of the spinal cord or sham laminectomy and then were allowed to recover on normal rat chow for 4 weeks and further on HFD for an additional 8 weeks. Spinal cord tissues harvested from the rats were processed for Affymetrix microarray and further transcriptomic analysis. Overall design: Male, Long Evans rats (400g) (Harlan, Indianapolis, IN) (N=10) werewere maintained on standard chow (#8640, Envigo, 3.0 kCal/g; 17% fat, 54% carbohydrate, 29% protein). Rats were assigned to either sham-laminectomy (Sham) or thoracic spinal cord injury (tSCI) group in a counterbalanced fashion based on body weight on the day prior to the start of surgery. Surgery was performed and animals were allowed to recover for 4 weeks following surgery. Rats were switched to a palatable, high-fat diet (HFD) (#D03082706, Research Diets, New Brunswick, NJ, 4.54 kCal/g; 40% fat, 46% carbohydrate, 15% protein) for the remainder of the study totaling 8 weeks.

INSTRUMENT(S): [RaGene-2_0-st] Affymetrix Rat Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Michael R Garrett  

PROVIDER: GSE102964 | GEO | 2018-01-01

SECONDARY ACCESSION(S): PRJNA399640

REPOSITORIES: GEO

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Publications

Chronic spinal cord changes in a high-fat diet-fed male rat model of thoracic spinal contusion.

Spann Redin A RA   Lawson William J WJ   Grill Raymond J RJ   Garrett Michael R MR   Grayson Bernadette E BE  

Physiological genomics 20170818 9


Individuals that suffer injury to the spinal cord can result in long-term, debilitating sequelae. Spinal cord-injured patients have increased risk for the development of metabolic disease, which can further hinder the effectiveness of treatments to rehabilitate the cord and improve quality of life. In the present study, we sought to understand the impact of high-fat diet (HFD)-induced obesity on spinal cord injury (SCI) by examining transcriptome changes in the area of the injury and rostral and  ...[more]

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