Transcriptomics,Genomics

Dataset Information

160

RNA-seq analysis of mRNAs and lncRNAs expression in chciken ALV-J-infected primary monocyte-derived macrophages (MDM) and uninfected control.


ABSTRACT: Purpose: The goals of this study are to investigate the differentially expressed mRNAs and lncRNAs between ALV-J infected MDM and uninfected MDM in chickens by Illumina deep sequencing. Methods: Total RNA from two ALV-J-infected MDM (designated: J3h_1, J3h_2, J36h_1 and J36h_2) and two uninfected MDM samples (designated: NC3h_1, NC3h_2, NC36h_1 and NC36h_2) was isolated by TRIzol following the manufacturer’s instruction at 3 h post infection (hpi) and 36 hpi. RNA samples were subjected to Illumina deep sequencing by Illumina Hiseq 2000. Results:Compared to the uninfected control, a total of 1568 and 550 up-regulated genes were identified in chicken MDM at 3 hpi and 36 hpi respectively, and 1227 and 397 down-regulated genes were identified at 3 hpi and 36 hpi, respectively.128 and 30 DE lncRNAs were identified in MDM at 3 hpi and 36 hpi, respectively. Conclusions: Strong immune response induced by ALV-J infection in MDM at 3 hpi. Many genes, lncRNAs involved in immune response such as PRRs signaling pathway and Jak-STAT signaling pathway at 3 hpi. Specifically, 78 ISGs expression significantly increased in ALV-J-infected MDM at 3 hpi. We speculated that host innate immune response could inhibit ALV-J replication in chicken MDM. These results provide valuable insights into the game of host antiviral immune response and ALV-J infection. Overall design: Chicken mRNAs and lncRNAs profiles of ALV-J infected MDM and uninfected MDM were generated by deep sequencing, using Illumina Hiseq 2000.

INSTRUMENT(S): Illumina HiSeq 2000 (Gallus gallus)

SUBMITTER: Min Feng  

PROVIDER: GSE103207 | GEO | 2017-08-29

SECONDARY ACCESSION(S): PRJNA400490

REPOSITORIES: GEO

Similar Datasets

| GSE103206 | GEO
1000-01-01 | S-EPMC6070742 | BioStudies
2015-01-01 | S-EPMC4434839 | BioStudies
2018-01-01 | S-EPMC6171863 | BioStudies
2016-01-01 | S-EPMC5078265 | BioStudies
| GSE63676 | GEO
2016-01-01 | S-EPMC4735322 | BioStudies
2017-01-01 | S-EPMC5725138 | BioStudies
2019-01-01 | S-EPMC6789757 | BioStudies
2016-01-01 | S-EPMC5127015 | BioStudies