Dataset Information


MiR-200b is associated with cisplatin sensitivity in bladder cancer cells

ABSTRACT: We aimed to clarify the role of miR-200b in cisplatin (CDDP) sensitivity in bladder cancer (BCa). CDDP resistant T24 cells (T24RC) were transfected with a miR mimic negative control (NC) or a miR-200b mimic, after which cells were treated with or without CDDP. We found that ectopic miR-200b expression re-sensitized the T24RC cells to CDDP. Gene expression microarray analysis revealed that the combination of miR-200b and CDDP affected genes involved in CDDP sensitivity and cytotoxicity. Overall design: CDDP resistant T24 cells (T24RC) were transfected with a miR mimic negative control (NC) or a miR-200b mimic using Lipofectamine RNAiMAX and incubated for 72 h. Transfectants were then treated with or without 1.6 μg/ml of CDDP for additional 72 h. Total RNA was extracted using a TRI Reagent.

INSTRUMENT(S): Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)

SUBMITTER: Hiromu Suzuki  

PROVIDER: GSE103250 | GEO | 2018-05-31


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Epigenetic silencing of miR-200b is associated with cisplatin resistance in bladder cancer.

Shindo Tetsuya T   Niinuma Takeshi T   Nishiyama Naotaka N   Shinkai Nobuo N   Kitajima Hiroshi H   Kai Masahiro M   Maruyama Reo R   Tokino Takashi T   Masumori Naoya N   Suzuki Hiromu H  

Oncotarget 20180511 36

In this study, we identified microRNAs (miRNAs) involved in cisplatin (CDDP) resistance in bladder cancer (BCa). After establishing CDDP-resistant BCa cell lines (T24RC and EJ138RC), TaqMan arrays revealed that members of the miR-200 family (miR-200b, miR-200a and miR-429) were downregulated in T24RC as compared to parental T24 cells. miR-200b was associated with CDDP sensitivity in BCa cells, and its downregulation was associated with CpG island hypermethylation. Pharmacological demethylation u  ...[more]

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