Genomics

Dataset Information

44

RNA sequncing of HCN3+ urinary tract pacemaker cells


ABSTRACT: Purpose: to analyze the mRNA content of highly purified HCN3+ urinary tract pacemaker cells compared to HCN3- neighboring cells Methods: we sorted HCN3+ and HCN3- cells from E18.5 WT embryos and analyzed their content using RNA-seq Results: Identified differentially expressed transcripts in HCN3+ cells compared to HCN3- cells Conclusions: our study presents the first whole transcriptome analysis of HCN3+ urinary tract pacemaker cells that would provide a basis for the charactarization of the development and function of those cells Overall design: RNA sequncing of HCN3+ urinary tract pacemaker cells in comparison to HCN3- cells

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

ORGANISM(S): Mus Musculus

SUBMITTER: Norman Rosenblum  

PROVIDER: GSE103400 | GEO | 2018-04-17

REPOSITORIES: GEO

Dataset's files

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GSE103400_HNC3-_vs_+.txt.gz Txt
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Publications

Protein Kinase 2<i>β</i> Is Expressed in Neural Crest-Derived Urinary Pacemaker Cells and Required for Pyeloureteric Contraction.

Iskander Samir M SM   Feeney Meghan M MM   Yee Kirby K   Rosenblum Norman D ND  

Journal of the American Society of Nephrology : JASN 20180207 4


Nonobstructive hydronephrosis, defined as dilatation of the renal pelvis with or without dilatation of the ureter, is the most common antenatal abnormality detected by fetal ultrasound. Yet, the etiology of nonobstructive hydronephrosis is poorly defined. We previously demonstrated that defective development of urinary tract pacemaker cells (utPMCs) expressing hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) and the stem cell marker cKIT causes abnormal ureteric peristalsis a  ...[more]

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