Transcriptomics,Genomics

Dataset Information

97

Transcriptome analysis of Wnt3a-treated Wild Type, Lrp5KO , Lrp6KO and Lrp5/6KO osteoblasts


ABSTRACT: Wnt signaling is a major regulator of osteoblast differentiation and function. To investigate how Wnt3a signaling regulates osteoblastic gene expression and to identify the role of Lrp5 and Lrp6 in mediating Wnt3a signaling in osteoblasts, neonatal calvarial osteoblasts isolated from C57Bl6 (WT) and osteoblasts lacking Lrp5 (Lrp5KO), Lrp6 (Lrp6KO) and, both Lrp5 and 6 (Lrp5/6KO) were treated with Wnt3a for 24 hours and gene expression changes were quantified by RNA-seq. Overall design: Transcriptome analysis to identify Wnt3a regulated genes in Wild Type, Lrp5KO , Lrp6KO and Lrp5/6KO osteoblasts

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Aimy Sebastian  

PROVIDER: GSE103492 | GEO | 2017-11-07

SECONDARY ACCESSION(S): PRJNA401759

REPOSITORIES: GEO

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Publications

Wnt co-receptors Lrp5 and Lrp6 differentially mediate Wnt3a signaling in osteoblasts.

Sebastian Aimy A   Hum Nicholas R NR   Murugesh Deepa K DK   Hatsell Sarah S   Economides Aris N AN   Loots Gabriela G GG  

PloS one 20171127 11


Wnt3a is a major regulator of bone metabolism however, very few of its target genes are known in bone. Wnt3a preferentially signals through transmembrane receptors Frizzled and co-receptors Lrp5/6 to activate the canonical signaling pathway. Previous studies have shown that the canonical Wnt co-receptors Lrp5 and Lrp6 also play an essential role in normal postnatal bone homeostasis, yet, very little is known about specific contributions by these co-receptors in Wnt3a-dependent signaling. We used  ...[more]

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