Transcriptomics,Genomics

Dataset Information

39

Root hair specific transcriptome analysis of gain-of-function and loss-of-function of GTL1 and DF1


ABSTRACT: How plants determine the final size of growing cells is an important, yet unanswered question. Root hairs provide an excellent model system to study this question since their final cell size is remarkably constant under given environmental conditions. In this study we demonstrate that a trihelix transcription factor GT-2-LIKE1 (GTL1) and its homolog DF1 repress root hair growth in Arabidopsis. Our transcriptional data, combined with genome-wide chromatin binding data, show that GTL1 and DF1 directly bind the RSL4 promoter and regulate its expression to repress root hair growth. Our data further show that GTL1 and RSL4 regulate each other as well as a set of common downstream genes, many of which have previously been implicated in root hair growth. This study therefore uncovers a core regulatory module that fine-tunes the extent of root hair growth by orchestrated actions of opposing transcription factors. Overall design: Seedlings were grown for 5 days. Nuclear of root hairs were isolated by GFP fluorecent (pEXPA7:NLS-GFP, pEXPA7:GTL1-GFP, or pEXPA7:DF1-GFP). Subsequently, total RNA was extracted from the isolated nuclear.

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: Michitaro Shibata  

PROVIDER: GSE103917 | GEO | 2018-02-15

REPOSITORIES: GEO

altmetric image

Publications

GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis.

Shibata Michitaro M   Breuer Christian C   Kawamura Ayako A   Clark Natalie M NM   Rymen Bart B   Braidwood Luke L   Morohashi Kengo K   Busch Wolfgang W   Benfey Philip N PN   Sozzani Rosangela R   Sugimoto Keiko K  

Development (Cambridge, England) 20180208 3


How plants determine the final size of growing cells is an important, yet unresolved, issue. Root hairs provide an excellent model system with which to study this as their final cell size is remarkably constant under constant environmental conditions. Previous studies have demonstrated that a basic helix-loop helix transcription factor ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4) promotes root hair growth, but how hair growth is terminated is not known. In this study, we demonstrate that a trihelix trans  ...[more]

Similar Datasets

2012-12-04 | E-MTAB-4375 | ArrayExpress
2014-08-05 | E-GEOD-38486 | ArrayExpress
| GSE111350 | GEO
2005-01-19 | E-NASC-54 | ArrayExpress
| GSE90142 | GEO
2007-01-22 | GSE6165 | GEO
2008-06-14 | E-GEOD-6165 | ArrayExpress
| GSE107699 | GEO
2012-12-14 | E-GEOD-42899 | ArrayExpress
2016-06-01 | E-GEOD-67921 | ArrayExpress