Transcriptomics,Genomics

Dataset Information

47

Expression data from keratinocytes treated either with IL-22 or combination of tofacitinib (JAK1/3 inhibitor) and IL-22 at 6 hours


ABSTRACT: JAK inhibitors like tofacitinib were thought to act primarily on T cells. However, our data and recent research suggest that JAK receptors are also present on keratinocytes. Here, we show effect of tofacitinib on primary keratinocytes, which could explain effects of topical tofacitinib treatment in psoriasis. We have performed whole transcriptome analysis (using microarray) on RNA isolated from cultured primary keratinocytes treated either with IL-22 alone or combination of tofacitinb and IL-22 at 6 hours Overall design: Primary keratinocytes were cultured in-vitro and pretreated with tofacitinib (0.6μM) for 1 hour followed by IL-22 (20 ng/ml) treatment for 6 hours. Total RNA was isolated and whole transcriptomic analysis was performed.

INSTRUMENT(S): [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]

SUBMITTER: Ankit Srivastava  

PROVIDER: GSE104509 | GEO | 2017-10-03

SECONDARY ACCESSION(S): PRJNA412876

REPOSITORIES: GEO

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Publications

Tofacitinib Represses the Janus Kinase-Signal Transducer and Activators of Transcription Signalling Pathway in Keratinocytes.

Srivastava Ankit A   Ståhle Mona M   Pivarcsi Andor A   Sonkoly Enikö E  

Acta dermato-venereologica 20180801 8


Tofacitinib is a Janus kinase (JAK) inhibitor, which has shown efficacy in treating psoriasis. The mode of action of tofacitinib is not completely understood but it has been thought to be mediated by the inhibition of CD4+ T-cell activation. Here, we investigated whether the molecular targets of tofacitinib are expressed in keratinocytes, and whether tofacitinib can modulate the activity of the JAK/Signal Transducer and Activators of Transcription (STAT)-pathway in keratinocytes. Transcriptomic  ...[more]

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