Genomics

Dataset Information

52

Zinc transporter ZIP8 (SLC39A8) overexpression effect on primary mouse articular chondrocytes


ABSTRACT: Gene expression profiling of primary mouse articular chondrocyte infected with recombinant adenovirus expressing the zinc transporter ZIP8 (SLC39A8) protein. In this study, we have attempted to explore the effects of ZIP8 overexpression on mouse transcriptome and have identified numerous genes which are involved in osteoarthritis pathogenesis. Overall design: Primary mouse articular chondrocytes were isolated from mouse femoral condyles and tibial plateaus cartilage tissue (WT ICR 5-day-old mice) and were infected with null(control) or recombinant adenovirus expressing zinc transporter ZIP8 for 24 hours. At 24 hours post-infection, total RNA was extracted from infected with null(control) adenovirus (4 biological replicates) or infected with recombinant adenovirus expressing the zinc transporter ZIP8 (4 biological replicates) on primary mouse articular chondrocytes and subjected to microarray analysis.

INSTRUMENT(S): [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Yoonkyung Won  

PROVIDER: GSE104795 | GEO | 2017-10-10

SECONDARY ACCESSION(S): PRJNA413833

REPOSITORIES: GEO

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Publications

Pleiotropic roles of metallothioneins as regulators of chondrocyte apoptosis and catabolic and anabolic pathways during osteoarthritis pathogenesis.

Won Yoonkyung Y   Shin Youngnim Y   Chun Churl-Hong CH   Cho Yongsik Y   Ha Chul-Won CW   Kim Jin-Hong JH   Chun Jang-Soo JS  

Annals of the rheumatic diseases 20160222 11


<h4>Objective</h4>The zinc-ZIP8-MTF1 axis induces metallothionein (MT) expression and is a catabolic regulator of experimental osteoarthritis (OA) in mice. The main aim of the current study was to explore the roles and underlying molecular mechanisms of MTs in OA pathogenesis.<h4>Methods</h4>Experimental OA in mice was induced by destabilisation of the medial meniscus or intra-articular injection of adenovirus carrying a target gene (Ad-Zip8, Ad-Mtf1, Ad-Epas1, Ad-Nampt, Ad-Mt1 or Ad-Mt2) into w  ...[more]

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