Transcriptomics

Dataset Information

156

H.pylori infections induces a transcriptional signature in gastric eosinophils, as determined by RNA sequencing


ABSTRACT: Eosinophils are highly abundant throughout the gastrointestinal tract. However, their function in bacterially-induced gastrointestinal inflammatory disorders is poorly understood. In this study, we have examined the function and regulation of gastric eosinophils in the steady state and during infection with Helicobacter pylori. We find that eosinophils are recruited to sites of infection, make direct contact with live bacteria, and activate a signature transcriptional program. Overall design: SiglecF+ CD11b+ MHCII- gastric eosinophils were sorted by flow cytometry from the gastric lamina propria of individual H. pylori-infected B6 mice or uninfected controls and compared for their gene expression profile.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Hubert Rehrauer  

PROVIDER: GSE105143 | GEO | 2018-06-29

REPOSITORIES: GEO

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Publications

Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation.

Arnold Isabelle C IC   Artola-Borán Mariela M   Tallón de Lara Paulino P   Kyburz Andreas A   Taube Christian C   Ottemann Karen K   van den Broek Maries M   Yousefi Shida S   Simon Hans-Uwe HU   Müller Anne A  

The Journal of experimental medicine 20180703 8


Eosinophils are predominantly known for their contribution to allergy. Here, we have examined the function and regulation of gastrointestinal eosinophils in the steady-state and during infection with <i>Helicobacter pylori</i> or <i>Citrobacter rodentium</i> We find that eosinophils are recruited to sites of infection, directly encounter live bacteria, and activate a signature transcriptional program; this applies also to human gastrointestinal eosinophils in humanized mice. The genetic or anti-  ...[more]

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