Transcriptomics,Genomics

Dataset Information

94

Transcriptome of Melampsora larici-populina sexual stages


ABSTRACT: Illumina HiSeq2500 technology was used to generate mRNA-sequencing expression profiles of sexual stages of the poplar rust fungus M. larici-populina: - Basidia (BSD) were collected from rust-infected dead poplar leaves; - Pycnia (PYC) were collected from rust-infected larch needles one week after inoculation with basidiospores and corresponded to different haploid fungal cell types such as in planta infection hyphae, receptive hyphae and pycniospores; - Aecia (AEC) were collected from rust-infected larch needles two weeks after inoculation, and corresponded to freshly produced aecia and dikaryotic aeciospores and remaining pycnia. Paired-end reads of 100bp were generated for three sets of biological replicates corresponding to each of the targeted stage and aligned to the genome of M. larici-populina isolate 98AG31 (version 1.0; US Department of Energy Joint Genome Institute; http://genome.jgi.doe.gov/Mellp1/Mellp1.home.html; Duplessis et al. 2011a) using CLC Genomics Workbench 8.0.1 Overall design: In total, nine mRNA profiles of M. larici-populina sexual stages were generated by Illumina HiSeq2500 (2x100bp). Three biological replicates were sequenced for each stage BSD, PYC and AEC.

INSTRUMENT(S): Illumina HiSeq 2500 (Melampsora larici-populina)

SUBMITTER: Sebastien Duplessis 

PROVIDER: GSE106863 | GEO | 2018-01-15

SECONDARY ACCESSION(S): PRJNA418247

REPOSITORIES: GEO

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Publications

The Rust Fungus Melampsora larici-populina Expresses a Conserved Genetic Program and Distinct Sets of Secreted Protein Genes During Infection of Its Two Host Plants, Larch and Poplar.

Lorrain Cécile C   Marchal Clémence C   Hacquard Stéphane S   Delaruelle Christine C   Pétrowski Jérémy J   Petre Benjamin B   Hecker Arnaud A   Frey Pascal P   Duplessis Sébastien S  

Molecular plant-microbe interactions : MPMI 20180606 7


Mechanisms required for broad-spectrum or specific host colonization of plant parasites are poorly understood. As a perfect illustration, heteroecious rust fungi require two alternate host plants to complete their life cycles. Melampsora larici-populina infects two taxonomically unrelated plants, larch, on which sexual reproduction is achieved, and poplar, on which clonal multiplication occurs, leading to severe epidemics in plantations. We applied deep RNA sequencing to three key developmental  ...[more]

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