Transcriptomics,Genomics

Dataset Information

43

Identification of rust-induced genes in poplar using Populus PICME 28K cDNA microarrays


ABSTRACT: Microarray expression profiling was carried out in poplar leaves upon infection with rust in order to identify genes expressed during tree defense response. For this purpose, we inoculated detached leaves of the interamerican hybrid poplar Populus trichocarpa x Populus deltoides 'Beaupré' grown in greenhouse with spores of avirulent strain 93ID6 of the pathogenic rust fungus Melampsora larici-populina (incompatible interaction, I48). Besides, we mock-inoculated 'Beaupré' leaves with water (control condition, T48). Detached leaves were maintained in vitro in controled conditions to allow fungal infection and colonization of plant tissue. Leaves were sampled 48 hours post-inoculation after that the fungus attempt to penetrate plant cells in mesophyll. Competitive hybridization between transcripts of incompatible interaction (I48) and control condition (T48) was done on Populus PICME 28K cDNA microarray. Keywords: Time-course infection of plant tissue, defense response, cDNA microarray Overall design: Three biological replicates of comparison between I48 and T48 treatments were tested, and one was discarded because of post-processing hybridization problems. Comparison between the two biological replicates served to identify consistent expression data in this experiment.

INSTRUMENT(S): PICME Poplar 28K 4_2_5

SUBMITTER: Sebastien Duplessis 

PROVIDER: GSE7049 | GEO | 2007-03-15

SECONDARY ACCESSION(S): PRJNA98511

REPOSITORIES: GEO

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Publications

Transcript profiling of poplar leaves upon infection with compatible and incompatible strains of the foliar rust Melampsora larici-populina.

Rinaldi Cécile C   Kohler Annegret A   Frey Pascal P   Duchaussoy Frédéric F   Ningre Nathalie N   Couloux Arnaud A   Wincker Patrick P   Le Thiec Didier D   Fluch Silvia S   Martin Francis F   Duplessis Sébastien S  

Plant physiology 20070330 1


To understand key processes governing defense mechanisms in poplar (Populus spp.) upon infection with the rust fungus Melampsora larici-populina, we used combined histological and molecular techniques to describe the infection of Populus trichocarpa x Populus deltoides 'Beaupré' leaves by compatible and incompatible fungal strains. Striking differences in host-tissue infection were observed after 48-h postinoculation (hpi) between compatible and incompatible interactions. No reactive oxygen spec  ...[more]

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