Transcriptomics,Genomics

Dataset Information

165

Differential expression of microRNAs in monocytes and derived macrophages in culture.


ABSTRACT: HIV-1 infection of monocytes/macrophages is modulated by several host factors. For instance, although HIV-1 successfully enters monocytes, antiviral restriction factors inhibit HIV-1 productive infection in these cells. We recently identified two microRNAs, miR-221 and miR-222, which limit HIV-1 entry during infection of monocyte-derived macrophages (MDM) by down-regulating CD4 expression. Interestingly, CD4 is also down regulated during the differentiation of monocytes into macrophages. In this study, we compared microRNA expression in monocytes and macrophages by RNAseq and found that miR-221/miR-222 are enhanced in macrophages.Taken together, these results show that both miR-221 and miR-222 act as modulators of CD4 mRNA expression throughout the monocyte/macrophage lineage, having roles in their differentiation, activation processes and modulating macrophage resistance to HIV-1. Overall design: Macrophages derived from the blood of 2 different adults (n=2 adults) were used. MicroRNAseq was performed on monocytes and their corresponding derived macrophages.

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Eric A Cohen 

PROVIDER: GSE107160 | GEO | 2017-11-21

SECONDARY ACCESSION(S): PRJNA419204

REPOSITORIES: GEO

altmetric image

Publications

Regulation of CD4 Receptor and HIV-1 Entry by MicroRNAs-221 and -222 during Differentiation of THP-1 Cells.

Lodge Robert R   Gilmore Julian C JC   Ferreira Barbosa Jérémy A JA   Lombard-Vadnais Félix F   Cohen Éric A ÉA  

Viruses 20171230 1


Human immunodeficiency virus type-1 (HIV-1) infection of monocyte/macrophages is modulated by the levels of entry receptors cluster of differentiation 4 (CD4) and C-C chemokine receptor type 5 (CCR5), as well as by host antiviral restriction factors, which mediate several post-entry blocks. We recently identified two microRNAs, miR-221 and miR-222, which limit HIV-1 entry during infection of monocyte-derived macrophages (MDMs) by down-regulating CD4 expression. Interestingly, CD4 is also down-re  ...[more]

Similar Datasets

| GSE103394 | GEO
2009-03-01 | GSE14542 | GEO
2010-06-25 | E-GEOD-14542 | ArrayExpress
2018-07-05 | GSE89918 | GEO
| GSE108897 | GEO
2015-05-22 | E-GEOD-57676 | ArrayExpress
2008-12-01 | GSE10890 | GEO
2011-06-14 | E-GEOD-29327 | ArrayExpress
2011-12-14 | E-GEOD-34428 | ArrayExpress
2009-01-13 | E-GEOD-10890 | ArrayExpress