Genomics

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Identification of Glucocorticoid-Induced Leucine Zipper (Gilz) gene targets in undifferentiated spermatogonia

ABSTRACT: Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. We have previously demonstrated a critical cell-autonomous role for Gilz in spermatogonial stem cell maintainance and spermatogenesis. To identify genes regulated by Gilz in the male germline, we have isolated undifferentiated spermatogonial cells from tamoxifen treated Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) mice that will allow identification of genes mis-expressed upon loss of GILZ. Overall design: 4 independent sets of Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) undifferentiated spermatogonia were isolated by flow sorting from adult mouse testes 7 days after treatment with tamoxifen.

INSTRUMENT(S): Illumina HiSeq 3000 (Mus musculus)

SUBMITTER: Robin Mark Hobbs  

PROVIDER: GSE107893 | GEO | 2018-08-24

REPOSITORIES: GEO

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GSE107893_NonStrandedCounts-withNames-proteinCoding.txt.gz Txt
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Publications

GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance.

La Hue M HM   Chan Ai-Leen AL   Legrand Julien M D JMD   Rossello Fernando J FJ   Gangemi Christina G CG   Papa Antonella A   Cheng Qiang Q   Morand Eric F EF   Hobbs Robin M RM  

Development (Cambridge, England) 20180918 18

Male fertility is dependent on spermatogonial stem cells (SSCs) that self-renew and produce differentiating germ cells. Growth factors produced within the testis are essential for SSC maintenance but intrinsic factors that dictate the SSC response to these stimuli are poorly characterised. Here, we have studied the role of GILZ, a TSC22D family protein and spermatogenesis regulator, in spermatogonial function and signalling. Although broadly expressed in the germline, GILZ was prominent in undif  ...[more]

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