Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis reveals the importance of sugar metabolism and export for positive mycorrhizal growth responses in soybean

ABSTRACT: To elucidate molecular mechanisms controlling differential growth responses to root colonization by arbuscular mycorrhizal (AM) fungi varying in colonization and cooperative behavior, transcriptome analyses were carried out using two soybean genotypes and three AM inocula. The results showed that inoculation by cooperative Rhizophagus irregularis (Ri) or less-cooperative Glomus aggregatum with high AM colonization (Ga-H) significantly promoted plant growth compared with inoculation by Glomus aggregatum with low AM colonization (Ga-L). A large number of soybean genes, especially those involved in metabolism, transport and regulation of lipids were significantly up-regulated by these three AM inoculations compared with un-inoculated control plants. However, genes encoding two SWEET transporters and one invertase were only up-regulated in response to Ri and Ga-H inoculation, which coincided with positive growth responses in these two inoculation treatments. Promoter analyses in transgenic soybean plants further demonstrated that GUS driven by the GmSWEET6 promoter was highly expressed in arbuscule-containing cortical cells. Additionally, Ri and Ga-H inoculation increased the contents of sucrose, glucose and fructose in both shoots and roots. These results imply that positive mycorrhizal growth responses in plants may mostly be due to the stimulation of photosynthate metabolism and transport by AM fungal inoculum with high colonization capabilities.

ORGANISM(S): Glycine max

PROVIDER: GSE107970 | GEO | 2025/03/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel gene expression in Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on whole mycorrhizal roots. We used GeneChips to detail the global programme of gene expression in response to colonization by arbuscular mycorrhizal fungi and in response to a treatment with phosphate and identified genes differentially expressed during this process. Medicago truncatula roots were harvested at 28 days post inoculation with the two different arbuscular mycorrhizal fungi Glomus intraradices (Gi-Myc) and Glomus mosseae (Gm-Myc) under low phosphate conditions (20 µM phosphate) or after a 28 days treatment with 2 mM phosphate in the absence of arbuscular mycorrhizal fungi (2mM-P). As a control, uninfected roots grown under low phosphate conditions (20 µM phosphate) were used (20miM-P). Three biological replicates consisting of pools of five roots were used for RNA extraction and hybridization on Affymetrix GeneChips.

2011-10-28 | E-GEOD-32208 | biostudies-arrayexpress

A roadmap of cell-type specific gene expression during late stages of the arbuscular mycorrhiza symbiosis

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel cell-type specific gene expression during late stages of Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on laser-microdissected cells. We used Medicago GeneChips to detail the cell-type specific programme of gene expression in late stages of colonization by arbuscular mycorrhizal fungi and identified genes differentially expressed during these stages. Medicago truncatula Gaertn M-bM-^@M-^XJemalongM-bM-^@M-^Y genotype A17 plantlets were grown in the climate chamber. Plants grown for the collection of root cortical cells containing arbuscules (ARB), root cortical cells from mycorrhizal roots (CMR), and root epidermal cells from mycorrhizal roots (EPI) were mycorrhized after 2 weeks with Glomus intraradices and mycorrhizal roots were harvested at around 21 days post inoculation (dpi).

2013-04-28 | E-GEOD-42747 | biostudies-arrayexpress

Transcriptome analysis of mycorrhizal and non-mycorrhizal soybean root associated with F. virguliforme inoculation

Project description:Many of the microorganisms that are normally present in the soil, actually inhabit the rhizosphere and interact with plants. Those plant–microorganisms interactions may be beneficial or harmful. Among the first are the arbuscular mycorrhizal fungi (AMF). These soil fungi have been reported to improve plant resistance/tolerance to pests and diseases. On the other hand, soilborne pathogens represent a threat to agriculture generating important yield losses, depending upon the pathogen and the crop. One example is the “Sudden Death Syndrome” (SDS), a severe disease in soybean (Glycine max (L.) Merr) caused by a complex of at least four species of Fusarium sp., among which Fusarium virguliforme and F. tuccumaniae are the most prevalent in Argentina. This study provides, under strict in vitro culture conditions, a global analysis of transcript modifications in mycorrhizal and non-mycorrhizal soybean root associated with F. virguliforme inoculation. Microarray results showed qualitative and quantitative changes in the expression of defense-related genes in mycorrhizal soybean, suggesting that AMF are good candidates for sustainable plant protection against F. virguliforme.

2018-11-12 | GSE108964 | GEO

Gene expression data from Mt/Gi vs Mt root tissues

Project description:Most vascular flowering plants have the ability to form mutualistic associations with soil fungi from the Glomeromycota. The resulting symbiosis is called an arbuscular mycorrhiza and they are widespread in terrestrial ecosystems throughout the world. Significant alteration occurs at physiological and molecular levels in both symbionts. To gain a better understanding of the AM symbiosis, we use a 16000 feature oligonucleotide based array to examine gene expression in an arbuscular mycorrhizal symbioses, M. truncatula/G. intraradices. Keywords: Medicago truncatula, Mycorrhizal, Glomus intraradices, microarray profiling

2007-03-08 | GSE5467 | GEO

Transcriptional response of soybean plants to soybean mosaic virus infection

Project description:Transcriptional changes in soybean plants infected with Soybean mosaic virus (SMV) was assessed at 7, 14 and 21 days post inoculation (dpi). Keywords: Time course

2008-03-17 | GSE9824 | GEO

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

2011-10-29 | GSE32208 | GEO

Gene expression during early stages of arbuscular mycorrhizal root formation in Medicago truncatula.

2012-03-28 | GSE34617 | GEO

RNA-Seq maize B73

Project description:In this study, plants of maize inbred line B73 descended from two different sets of seed material grown for several generations either in the field or in the greenhouse were found to show a different growth phenotype and ionome under phosphate starvation conditions and moreover a different responsiveness towards mycorrhizal fungi of the species Glomus intraradices (syn: Rhizophagus irregularis).Transcriptome sequencing of three individuals from each set lent further support to the location of the introgression intervals and confirmed them to be fixed in all sequenced individuals. Moreover, we identified >120 genes differentially expressed between the two B73 lines.

2014-05-02 | E-MTAB-4285 | biostudies-arrayexpress

Transcriptome during autoregulation of mycorrhization in soybean

Project description:The autoregulation of mycorrhization (AOM) describes a plant regulatory mechanism that limits the number of infection events by arbuscular mycorrhizal fungi. The key signal mediator is a receptor kinase (GmNARK) that acts in the shoots. Early signals of the mycorrhizal symbiosis induce a root-derived signal that activates GmNARK in the shoot finally leading to a systemic repression of subsequent infections in the root. So far, less is known about the signals down-stream of GmNARK. To find genes regulated by GmNARK in a mycorrhiza-dependent as well as in a mycorrhiza-independent manner, we used the Affymetrix GeneChip for soybean. In general, mycorrhizal root systems consist of both colonized and non-colonized, but autoregulated roots. To physically separate those two root types for transcript analysis of specifically regulated genes, we used the split-root system. Transcript profiling during AOM was done with material of Bragg wild-type and of the nark mutant nts1007, either non-inoculated or partially inoculated with the mycorrhizal fungus Rhizophagus irregularis (formerly Glomus intraradices). Wild-type and nark mutants were inoculated with R. irregularis on one half of the root-systems (root-parts "A") only. The remaining half of the root-systems stayed non-infected (root-parts "B"). Corresponding controls stayed completely non-infected. Gene expression was analyzed in inoculated root-parts, non-inoculated root-parts and shoots of three individual plants per treatment. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Sara Schaarschmidt. The equivalent experiment is GM53 at PLEXdb.]

2013-05-09 | GSE44685 | GEO

Transcription profiling of Lycopersicon esculentum roots in wild-type and ABA-deficient, control and Glomus intraradices inoculated plants to characterise variations in mycorrhization.

Project description:Abscisic acid (ABA) determines mycorrhiza functionality and arbuscule development. Transcriptome analysis in response to different mycorrhization status according to the ABA concentration in the root was performed to identify genes that may play a role in arbuscule functionality. Tomato Affymetrix GeneChip (around 10,000 probes) allowed us to detect and compare the transcriptional root profiling of tomato (Solanum lycopersicum) wild-type and ABA-deficient sitiens plants colonized by the arbuscular mycorrhizal fungus Glomus intraradices. <br><br>

2010-07-01 | E-MEXP-2356 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data