Transcriptomics,Genomics

Dataset Information

42

Transcriptional response of soybean plants to soybean mosaic virus infection


ABSTRACT: Transcriptional changes in soybean plants infected with Soybean mosaic virus (SMV) was assessed at 7, 14 and 21 days post inoculation (dpi). Keywords: Time course Overall design: Two separate cDNA labeling reactions (Cy3 and Cy5), one for the RNA from SMV-infected leaf tissues and the other for the RNA from the mock-inoculated leaves, were carried out. A total of 18 microarray slides were used for this study: 6 array hybridizations including three reciprocal labeling experiments from three independent biological replications of either SMV-infected or mock-inoculated leaf at each of the three time points. Dye swaps were performed to ensure that the results were not biased by dye effects.

INSTRUMENT(S): Soybean 18k-A array

SUBMITTER: Mohan babu  

PROVIDER: GSE9824 | GEO | 2008-03-17

SECONDARY ACCESSION(S): PRJNA103787

REPOSITORIES: GEO

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Publications

Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection.

Babu Mohan M   Gagarinova Alla G AG   Brandle James E JE   Wang Aiming A  

The Journal of general virology 20080401 Pt 4


Compatible virus infection induces and suppresses host gene expression at the global level. These gene-expression changes are the molecular basis of symptom development and general stress and defence-like responses of the host. To assess transcriptional changes in soybean plants infected with soybean mosaic virus (SMV), the first soybean trifoliate leaf, immediately above the SMV-inoculated unifoliate leaf, was sampled at 7, 14 and 21 days post-inoculation (p.i.) and subjected to microarray anal  ...[more]

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