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GmMEKK2 enhanced mosaic virus resistance in soybean (Glycine max L.)

ABSTRACT: Mitogen-activated protein kinase kinase kinase (MAPKKK) assume a pivotal position within the MAPK cascade signaling pathway that converts external stimuli into intracellular responses, and plays a central role in adaptation and resistance to biotic and abiotic stress. Only a limited number of researches have reported that MAPKKK conducted on regulating the resistance to soybeans mosaic viruses (SMV). Here, we identified a MAPKKK 2-like gene named as GmMEKK2 from SMV resistance cultivar. Overexpression of GmMEKK2 in soybean not only reduced SMV content but also decreased the disease index of virus. Importantly, overexpression of GmMEKK2 mitigated the yield loss after SMV inoculation and improved the yield-related traits of soybean. These demonstrated that GmMEKK2 had a favorable role in SMV resistance. This study explored the functions of GmMEKK2 in soybean and provided an assertive solution for effectively improving SMV resistance.

ORGANISM(S): Glycine max

PROVIDER: GSE288208 | GEO | 2025/06/01

REPOSITORIES: GEO

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Project description:Resistance (R) genes are very effective for disease control in plants. Current research shows that R genes predominantly function by inducing a hypersensitive reaction (HR), which results in localized cell death thought to restrict pathogen spread. Some R genes elicit a more atypical response where resistance is not associated with HR or the associated gene expression changes, termed extreme resistance (ER). The molecular regulatory mechanism underlying ER is largely unexplored. One of the few known, naturally occurring, instances of ER is resistance derived from the soybean Rsv3 gene, which confers resistance against the most virulent strains of soybean mosaic virus (SMV). To discern the regulatory mechanism underlying the Rsv3-mediated ER reaction, we generated a gene regulatory network using transcriptomic data from a time course comparison of SMV-G7-infected resistant (L29, Rsv3-genotype) and susceptible (Williams82, rsv3-genotype) soybean cultivars. We identified putative interactions between transcription factors (TFs) regulating gene expression in hormone regulatory pathways, such as abscisic acid (ABA) and jasmonic acid (JA). This is consistent with the demonstrated involvement of these pathways in Rsv3-mediated resistance. We found significant enrichment for the G-box motif (“CACGTG”) among genes implicated in ABA- and JA-related activities. This motif is specifically recognized by MYC2, which is a master regulator of ABA and JA signaling. Our network identified a MYC2 TF encoded by Glyma.07G051500 as a putative transcriptional regulator whose expression was significantly down-regulated in L29. This correlated with the down-regulation of expression of genes involved in ABA and JA processes. Our results suggest an important function for Glyma.07G051500 in ABA and JA derived defense signaling. Additionally, our regulatory network found other putative TFs with differential expression, such as MYBs and ERFs, which may also be involved in regulating ABA and JA signaling for defense. The regulatory network presented here offers new insights into the regulation of the molecular defense mechanism underlying Rsv3-mediated ER against SMV.

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GmMEKK2 enhanced mosaic virus resistance in soybean (Glycine max L.)

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