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Expression data from PDGF-B induced murine gliomas transplanted in NOD/SCID mice

ABSTRACT: The different phases of tumor immunoediting in vivo were dissected thanks to a murine model of glioma induced by PDGF-B overexpression. We show that low-grade gliomas are highly immunostimulatory and that the adaptive immune system prevents the development of secondary tumor in syngeneic mice. During tumor progression, glioma cells downregulate immunostimulatory genes and the immune infiltrate becomes pro-tumorigenic. We showed that glioma cells are able to progress towards a high-grade phenotype even in immunodeficient mice, albeit more slowly and this progression invariably requires a downregulation of immunostimulatory genes. Overall design: Cells derived from early onset / low grade murine gliomas induced with PDGF-B overexpression were serially transplanted in immunodeficient NOD/SCID mice where they can root. Upon the development of secondary and tertiary gliomas, tumor cells were explanted, dissociated and sorted by FACS, basing on the expression of the EGFP transgene expressed togheter with PDGF-B oncogene as a bicistronic transcript. EGFP-positive cells were then either processed for RNA estraction.

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

SUBMITTER: Paolo Malatesta  

PROVIDER: GSE108955 | GEO | 2018-10-22


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Progression from low- to high-grade in a glioblastoma model reveals the pivotal role of immunoediting.

Appolloni Irene I   Alessandrini Francesco F   Ceresa Davide D   Marubbi Daniela D   Gambini Eleonora E   Reverberi Daniele D   Loiacono Fabrizio F   Malatesta Paolo P  

Cancer letters 20181009

The mutual reshape of tumor and immune system cells during tumor progression is a widely accepted notion in different cancers including gliomas. The importance of this phenomenon in shaping glioma progression and the mechanisms governing it, however, are not fully elucidated. Taking advantage of a well-characterized in vivo glioma model we performed an analysis of glioma cells transcriptomes at different stages of progression and unveiled the reorganization of glioma-immune system interactions.  ...[more]

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