Genomics

Dataset Information

36

Genome-wide maps of histone modification and chromatin remodeler binding in acute myeloid leukemia blast cells and normal hematopoietic cells


ABSTRACT: We have used chromatin immunoprecipitation followed by high throughput sequencing to map regions of chromatin remodeler binding accompanied by histone modification state. We have used this data to distinguish between the transcriptional state of regulatory genes in leukemic and normal hematopoietic cells, and thus understand contribution of the chromatin remodeler towards leukemogenesis. Overall design: Binding profiles of 2 different histone modifications, H3K27ac and H3K27me3, and the core ATP-ase subunit of the SWI/SNF chromatin remodeler, in duplicate, in blast cells derived from 3 different AML patients, and normal hematopoietic cells derived from 1 healthy individual, used as the control.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Amitava Sengupta  

PROVIDER: GSE108976 | GEO | 2018-01-10

SECONDARY ACCESSION(S): PRJNA429274

REPOSITORIES: GEO

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Publications

SMARCB1 Deficiency Integrates Epigenetic Signals to Oncogenic Gene Expression Program Maintenance in Human Acute Myeloid Leukemia.

Chatterjee Shankha Subhra SS   Biswas Mayukh M   Boila Liberalis Debraj LD   Banerjee Debasis D   Sengupta Amitava A  

Molecular cancer research : MCR 20180226 5


SWI/SNF is an evolutionarily conserved multi-subunit chromatin remodeling complex that regulates epigenetic architecture and cellular identity. Although <i>SWI/SNF</i> genes are altered in approximately 25% of human malignancies, evidences showing their involvement in tumor cell-autonomous chromatin regulation and transcriptional plasticity are limiting. This study demonstrates that human primary acute myeloid leukemia (AML) cells exhibit near complete loss of SMARCB1 (BAF47 or SNF5/INI1) and SM  ...[more]

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