Affinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi

ABSTRACT: Chromatin remodeling is a general mechanism involved in DNA-dependent processes, including gene expression regulation. ISWI ATPase is the catalytic subunit in diverse chromatin remodeling complexes that changing histone–DNA interactions and therefore play a pivotal role in different nuclear processes. Here, the ISWI remodeler was fused to GFP (NH and CO fusions) and its partners were determined by affinity purification-mass spectrometry (MS) assay. This approach identified known ISWI partners (NLP, RCCP and FYRP), previously characterized in T. brucei, and new components of TcISWI complex (DRBD2, DHH1 and proteins containing SMC domains).

INSTRUMENT(S):

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: Michel Batista  

LAB HEAD: Fabricio Klerynton Marchini

PROVIDER: PXD017869 | Pride | 2020-05-22

REPOSITORIES: Pride