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Affinity Purification of ISWI chromatin remodeler in Trypanosoma cruzi

ABSTRACT: Chromatin remodeling is a general mechanism involved in DNA-dependent processes, including gene expression regulation. ISWI ATPase is the catalytic subunit in diverse chromatin remodeling complexes that changing histone–DNA interactions and therefore play a pivotal role in different nuclear processes. Here, the ISWI remodeler was fused to GFP (NH and CO fusions) and its partners were determined by affinity purification-mass spectrometry (MS) assay. This approach identified known ISWI partners (NLP, RCCP and FYRP), previously characterized in T. brucei, and new components of TcISWI complex (DRBD2, DHH1 and proteins containing SMC domains).

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Trypanosoma Cruzi Dm28c

SUBMITTER: Michel Batista

LAB HEAD: Fabricio Klerynton Marchini

PROVIDER: PXD017869 | Pride | 2020-05-22

REPOSITORIES: Pride

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Characterising ISWI chromatin remodeler in Trypanosoma cruzi.

Díaz-Olmos Yirys Y Batista Michel M Ludwig Adriana A Marchini Fabricio K FK

Memorias do Instituto Oswaldo Cruz 20200518

BACKGROUND Imitation SWItch (ISWI) ATPase is the catalytic subunit in diverse chromatin remodeling complexes. These complexes modify histone-DNA interactions and therefore play a pivotal role in different DNA-dependent processes. In Trypanosoma cruzi, a protozoan that controls gene expression principally post-transcriptionally, the transcriptional regulation mechanisms mediated by chromatin remodeling are poorly understood. OBJECTIVE To characterise the ISWI remodeler in T. cruzi (TcISWI). METHO ...[more]

PMID: 32428081

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