Genomics

Dataset Information

44

Preferential localization of human origins of DNA replication at the 5' end of expressed genes


ABSTRACT: Human DNA replication relies on the activation of thousands of origins distributed along the genome. Their organization and the functional significance of their distribution remains unknown. To map large number of origins in human cells, we have localized the distribution of short nascent DNA using a high-resolution DNA tiling array platform covering 33.9Mb. Results with three different cell lines reveal that origins are very closely spaced (average interval 3-5kb), and that their positions are largely conserved among the cell lines studied. Origins are non-randomly distributed, being preferentially enriched at the 5’-end of expressed genes and at evolutionary intergenic sequences. In MCF7 cells, a strong correlation is also found between origin positioning and histone H3K4me3 and of PolII binding to chromatin. This study provides a large scale view of the organization of DNA replication origins in human chromosomes and suggests a link between this distribution and underlying chromatin features related to chromosome structure, and gene expression Keywords: nascent strand DNA, histone, Pol-II ChIP-chip. Overall design: To map initiation sites for DNA replication and the position of both H3K4Me3 and Pol-II chromatin binding sites in human cell lines on a custom made high density tiling DNA microarray covering 33.9 Mb of the human genome.

INSTRUMENT(S): Nimblegen 385K custom array (Human tiling array of hg16 at 50bp resolution (chr17q, chr20q, etc)).

ORGANISM(S): Homo Sapiens

SUBMITTER: Yuelin Jack Zhu  

PROVIDER: GSE10917 | GEO | 2011-05-13

SECONDARY ACCESSION(S): PRJNA107217

REPOSITORIES: GEO

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