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Analysis of gene expression during mouse preimplantation development in vitro, with or without a background of exogenous ovarian stimulation (superovulation)

ABSTRACT: Among the different procedures of the assisted reproductive technologies (ARTs), the purpose of ovarian stimulation with gonadotropins is to maximize the number of oocytes retrieved, and facilitate scheduling and planning of patient treatment. However, the doses of the gonadotropins required to increase the number of growing ovarian follicles, and, thereby, the number of oocytes, have been found to affect the quality of embryos in the mouse model of human reproduction (PMID 16569317, PMID 22802074, PMID 11387298, PMID 11157810). In order to clarify this issue, we compared the gene expression of mouse embryos with vs without a background of ovarian stimulation with PMSG and hCG. Pronuclear oocytes were cultured in KSOM(aa) and developmental stages were collected based on morphology. Analysis of transcriptome signal intensities showed similar profiles and produced the expected clustering (one cluster from oocyte to 2-cell stage, another cluster from 4-cell stage to blastocyst) regardless of whether the embryos were produced in stimulated or non-stimulated ovarian cycles; however, the expression levels of some genes were different depending on the ovarian stimulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE110599 | GEO | 2020/04/14

REPOSITORIES: GEO

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The trade-off hypothesis of oocyte quantity and quality in gonadotropin-stimulated ovarian regime: proteome profile of superovulated vs naturally ovulated mouse oocytes

Project description:In order to clarify if there is a tradeoff between quantity and quality of oocytes retrieved after ovarian stimulation, we compared the protein expression of mouse oocytes and embryos with vs without a background of ovarian stimulation using gonadotropins, administered according to the standard protocol in use since more than 50 years (PMID 13185277; PMID 13475597). The data we gathered contribute to a more complete understanding of embryonic gene expression, by taking into account the effect of the commonly used superovulation.

2021-12-14 | PXD026347 | Pride

The trade-off hypothesis of oocyte quantity and quality in gonadotropin-stimulated ovarian regime: proteome profile of superovulated vs naturally ovulated mouse oocytes

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Incorporation of isotopically labeled amino acids in live mouse zygotes as means to understand the oocyte to embryo transition

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2023-10-19 | PXD035570 | Pride

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Project description:In their attempt to fulfill the wish of having children, women who suffer from fertility issues often undergo assisted reproductive technologies (ART) such as ovarian stimulation, which has been associated with adverse health outcomes and imprinting disorders in children. However, given the crucial role of exogenous hormone stimulation in improving human infertility treatments, a more comprehensive analysis of the potential impacts on DNA methylation in embryos following ovarian stimulation is needed. Here, we provide genome-wide DNA methylation profiles of blastocysts generated after superovulation of prepubertal or adult mice, compared with blastocysts derived from non-stimulated adult mice. Additionally, we assessed the impact of the in vitro growth and maturation of oocytes on methylation in blastocysts.

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2014-11-15 | E-GEOD-53662 | biostudies-arrayexpress

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Project description:Four groups of human oocytes from in vitro fertilization programme were analysed using microarrays: 1.) oocytes matured in vivo (retrieved as mature by ultrasound follicular aspiration but not fertilized after in vitro fertilization), 2.) oocytes matured in vitro in a conventional way (in a maturation medium with added gonadotropins FSH and HCG), 3.) oocytes matured in vitro by a new approach (in a maturation medium with added gonadotropins FSH and HCG, and in a co-culture with cumulus cells from mature oocytes of the same patient), and 4.) not matured oocytes which did not mature in spite of in vitro maturation procedure. Each group of oocytes consisted of three biological replicates with 10 oocytes per replicate therefore 12 oocyte samples and altogether 120 human oocytes were analyzed by microarrays.

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