Dataset Information

Delignification abilities of the white-rot fungus Polyporus brumalis explained by its oxidative enzymatic arsenal

ABSTRACT: The fungus Polyporus brumalis is a wood decay fungus previously evidenced as efficient lignin degrader with high potential for plant biomass pre-treatment before conversion into bio-energy. Here we used an RNASeq approach that highlighted the active transcription of an unparalleled number of lignin active peroxidases and H2O2 generating enzymes during growth on wheat straw. These enzymes, together with metabolic processes related to detoxification appear as key determinants of the fungal adaption to lignin degradation. Overall design: To identify the enzymatic determinants responsible for the observed selective degradation of lignin, we compared the transcription profiles of P. brumalis after 10-day growth on 2% malt extract (control) with the transcription profiles after 6-days growth on 2% malt extract followed by 4, 10 and 15 days fermentation on wheat straw.

INSTRUMENT(S): Illumina HiSeq 2000 (Polyporus brumalis)

ORGANISM(S): Polyporus brumalis

SUBMITTER: Marie-Noëlle Rosso

PROVIDER: GSE112011 | GEO | 2018-12-21

REPOSITORIES: GEO

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			Action	DRS
	GSE112011_Polbr_RawCount.csv.gz	Csv

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Publications

Integrative visual omics of the white-rot fungus <i>Polyporus brumalis</i> exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass.

Miyauchi Shingo S Rancon Anaïs A Drula Elodie E Hage Hayat H Chaduli Delphine D Favel Anne A Grisel Sacha S Henrissat Bernard B Herpoël-Gimbert Isabelle I Ruiz-Dueñas Francisco J FJ Chevret Didier D Hainaut Matthieu M Lin Junyan J Wang Mei M Pangilinan Jasmyn J Lipzen Anna A Lesage-Meessen Laurence L Navarro David D Riley Robert R Grigoriev Igor V IV Zhou Simeng S Raouche Sana S Rosso Marie-Noëlle MN

Biotechnology for biofuels 20180723

<h4>Background</h4>Plant biomass conversion for green chemistry and bio-energy is a current challenge for a modern sustainable bioeconomy. The complex polyaromatic lignin polymers in raw biomass feedstocks (i.e., agriculture and forestry by-products) are major obstacles for biomass conversions. White-rot fungi are wood decayers able to degrade all polymers from lignocellulosic biomass including cellulose, hemicelluloses, and lignin. The white-rot fungus <i>Polyporus brumalis</i> efficiently brea ...[more]

PMID: 30061923

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